Soil aggregate stability and pore structure are key indicators of soil degradation. Waves generated by the water-level fluctuations could severely deteriorate soil aggregates, which eventually induce soil erosion and several other environmental issues such as sedimentation and flooding. However, due to limited availability of the hydrological alteration data, there is a limited understanding of soil aggregates, intra-aggregate pore dynamics, and their relationships under periodically flooded soils. The present study has relied on long-term hydrological alteration data (2006-2020) to explore the impacts of inundation and exposure on soil aggregates and pore structure variations. Soil samples from increasing elevations (155, 160, 163, 166, 169, and 172 m) in the water-level fluctuation zone of the Three Gorges Reservoir were exposed to wet-shaking stress and determined soil structural parameters. The overall inundation and exposure ratio (OvI/E) gradually decreased from 1.87 in the lowest to 0.27 in the highest elevation, respectively. Predominant distribution of macropores was recorded in lower elevations, while micropores were widely distributed in the upper elevations. The mean weight diameter (MWD) was significantly lower in the lower (2.4-3.7 mm) compared to upper (5.3-6.0 mm) elevations. The increase in MWD has increased the proportion of micropores (PoN < 50 mu m), with R-2 = 0.59. This could suggest that the decrease in flooding intensity can create favorable conditions for plant roots growth. The strong flooding stress in lower elevations (i.e., higher values of the OvI/E) accelerated the disintegration of soil aggregates and considerably increased the formation of macropores due to slaking and cracking. The findings of the present study emphasize the need to restore degraded soils in periodically submerged environments by implementing vegetation restoration measures. This could enhance and sustain aggregate stability, which was also proved to increase functional pores under hydrological alterations.

Biochar application, as a kind of soil amendment, significantly influences soil physical and mechanical properties. This study revealed the effects of biochar application on the physical and mechanical properties of a clay-type soil at different irrigation levels. Soil was treated with three levels of biochar application: B0 (0 t ha(-1)), B1 (25 t ha-(1)) and B2 (50 t ha b(-1)), and three levels of irrigation: T0 (1.2 pan evaporation Ep), T1 (1.0 Ep) and T2 (0.8 Ep). The results indicated that other treatments reduced the soil bulk density compared with the control treatment (CK) (B0T1). Compared to CK, the highest reduction in soil bulk density was 18%. Irrigation did not improve the soil bulk density and porosity at the same biochar application in the short term. Biochar enhanced the stability of the soil aggregates. Compared to CK, the largest MWD (mean weight diameter) was enhanced by 9%. The addition of biochar and decreasing irrigation could decrease soil cohesion. The addition of biochar and increasing irrigation could increase the soil internal friction angle. The soil cohesion first increased and then decreased as the soil water content increased. According to the fitting formula, the soil cohesion was found to be minimum at B2T2, which was a decrease of 39% compared to B0T1. At the same irrigation level, the soil internal friction angle decreased with increasing soil water content. Soil penetration resistance showed a decreasing trend with the application of biochar. The more irrigation there is, the larger the soil penetration resistance.

As the basic units of soil structure, soil aggregate is essential for maintaining soil stability. Intensified freeze-thaw cycles have deeply affected the size distribution and stability of aggregate under global warming. To date, it is still lacking about the effects of freeze-thaw cycles on aggregate in the permafrost regions of the Qinghai-Tibetan Plateau (QTP). Therefore, we investigated the effects of diurnal and seasonal freeze-thaw processes on soil aggregate. Our results showed that the durations of thawing and freezing periods in the 0-10 cm layer were longer than in the 10-20 cm layer, while the opposite results were observed during completely thawed and frozen periods. Freeze-thaw strength was greater in the 0-10 cm layer than that in the 10-20 cm layer. The diurnal freeze-thaw cycles have no significant effect on the size distribution and stability of aggregate. However, 0.25 mm) and reduced aggregate stability. Our study has scientific guidance for evaluating the effects of freeze-thaw cycles on soil steucture and provides a theoretical basis for further exploration on soil and water conservation in the permafrost regions of the QTP.